Fluge and Mella used an expensive bit of kit called the Seahorse analyser, which measures glycolysis through the lactate production and mitochondrial activity through changes in oxygen levels.
They tested normal healthy muscle cells that had been grown in the lab. But they added to those cells serum taken from either ME/CFS patients or healthy controls. Serum is the fluid left over after blood has clotted and it contains small molecules and other soluble substances.
They have data for 12 people with ME/CFS and 12 healthy controls, a relatively small sample.What they found was, surprisingly, that the muscle cells produced more lactate and burned more oxygen when they were incubated with ME/CFS serum than when incubated in serum from healthy controls. And the effect was particularly strong when the cells were made to work hard.
A sobering read on gentrification of downtown Raleigh from the New York Times.
RALEIGH, N.C. — In the African-American neighborhoods near downtown Raleigh, the playfully painted doors signal what’s coming. Colored in crimson, in coral, in seafoam, the doors accent newly renovated craftsman cottages and boxy modern homes that have replaced vacant lots.
To longtime residents, the doors mean higher home prices ahead, more investors knocking, more white neighbors.
Here, and in the center of cities across the United States, a kind of demographic change most often associated with gentrifying parts of New York and Washington has been accelerating. White residents are increasingly moving into nonwhite neighborhoods, largely African-American ones.
The article doesn’t say it but I will: fuck John Walker, Jr.
In 1968 one of the U.S. Navy’s nuclear submarines went missing in the Atlantic. Now, 50 years later, the full story of its disappearance can finally be told.RADIOMEN 2ND CLASS MIKE HANNON WALKED TO WORK WITH A PALPABLE SENSE OF UNEASE on the morning of May 23, 1968. As a communications specialist at Submarine Force Atlantic Headquarters, he was responsible for processing dozens of messages each day from submarines at sea, ranging from routine announcements to top-secret operational dispatches. But hours earlier, when his eight-hour shift had ended at midnight, Hannon feared that one of the submarines on his watch might be in trouble—or worse.
The Norfolk-based USS Scorpion, one of the Atlantic Fleet’s 19 nuclear attack submarines, had been scheduled to transmit a four-word “Check Report”—encrypted to prevent the Soviets from intercepting it—that meant, in essence, “Situation normal, proceeding as planned.” In this instance, the Skipjack-class submarine was returning to Norfolk after a three-month deployment to the Mediterranean Sea. Its standing orders called for a burst transmission every 24 hours that, when decrypted, read: “Check 24. Submarine Scorpion.” But the previous day no message had come clattering out of the secure teletypewriter that Hannon used. As he prepared to leave for the night, Hannon had briefed Radioman 2nd Class Ken Larbes, the petty officer coming on duty, about the overdue message. He then tapped on his supervisor’s office door and asked whether any late word had come in from the Scorpion. Warrant Officer John A. Walker Jr. silently shook his head no. Was this the first hint of an emergency, Hannon wondered, or merely a delayed transmission caused by mechanical problems or stormy weather conditions?
It’s been another dizzying few days in Washington, starting with yet another border controversy, as President Donald Trump threatened to bus unauthorized immigrants to sanctuary cities, and ending with the release of Special Counsel Robert Mueller’s report, which turned out to be far more damning than advertised by Trump’s attorney general.
These two very different stories have more in common than meets the eye. In each case, there’s a central tension between the president and aides who refuse to execute orders from him that they believe are illegal or foolish. Mueller’s report is packed with incidents in which White House staff not only didn’t do things Trump said, but never had any intention of doing them. In the case of the border, Immigration and Customs Enforcement staff rebuffed Trump’s plan to bus migrants on legal grounds; meanwhile, Homeland Security Secretary Kirstjen Nielsen and Customs and Border Protection Commissioner Kevin McAleenan refused to turn away migrants seeking asylum, concluding that it was illegal. (Nielsen was sacked soon after, while McAleenan is now her acting replacement.)
It is difficult to exaggerate just what a sea change has taken place in the discussion of renewable energy in recent years.
Oldsters like me remember when the idea that (unsubsidized) renewable energy would be able to compete directly with fossil fuels was downright utopian. As late as the early 2000s, people were debating whether it would happen this century, or at all.
But the extraordinary progress of renewables in the past two decades has moved that hoped-for future closer and closer. And now, unbelievably, it is right on our doorstep.
It’s one thing for advocates or energy analysts to say that, of course. It’s something else to hear it coming out of the mouths of energy executives. But these days, residents of the C-suite are discussing renewable energy in terms that would have made hippies blush a decade ago.
In the end, the man who reportedly smeared feces on the walls of his lodgings, mistreated his kitten, and variously blamed the ills of the world on feminists and bespectacled Jewish writers was pulled from the Ecuadorian embassy looking every inch like a powdered-sugar Saddam Hussein plucked straight from his spider hole. The only camera crew to record this pivotal event belonged to Ruptly, a Berlin-based streaming-online-video service, which is a wholly owned subsidiary of RT, the Russian government’s English-language news channel and the former distributor of Julian Assange’s short-lived chat show.
RT’s tagline is “Question more,” and indeed, one might inquire how it came to pass that the spin-off of a Kremlin propaganda organ and now registered foreign agent in the United States first arrived on the scene. Its camera recorded a team of London’s Metropolitan Police dragging Assange from his Knightsbridge cupboard as he burbled about resistance and toted a worn copy of Gore Vidal’s History of the National Security State.
More evidence that our reliance on cars is killing us.
If you want to be as healthy as possible, there are no treadmills or weight machines required. Don’t just take my word for it—look to the longest-lived people in the world for proof.
People in the world’s Blue Zones—the places around the world with the highest life expectancy—don’t pump iron, run marathons or join gyms.
Instead, they live in environments that constantly nudge them into moving without even thinking about it. This means that they grow gardens, walk throughout the day, and minimize mechanical conveniences for house and yard work.
In fact, Blue Zones researchers determined that routine natural movement is one of the most impactful ways to increase your life span, and a common habit among the world’s longest-lived populations.
By steamrolling local taxi operations in cities all over the world and cultivating cheerleaders in the business press and among Silicon Valley libertarians, Uber has managed to create an image of inevitability and invincibility. But the company just posted another quarter of jaw-dropping losses — this time over $1 billion, after $4.5 billion of losses in 2017. How much is hype and how much is real?
A thought-provoking piece on what’s killing San Francisco.
It’s not what celebrants want to hear when the champagne is exploding out of shaken bottles of Dom, the confetti is falling, and their stock is up 8.7 percent at the market’s close, but I have an announcement to make: San Francisco is past its prime and the fires of creation have abated.
With all the millionaires newly minted by Lyft’s IPO, and with those set to be minted by Uber’s and Palantir’s and AirBnB’s, you might expect this enclave to become the next Babylon of American capitalism. While our moralists in the media — Nellie Bowles, Emily Chang, et al. — busily tsk-tsk the greed and the lust and the hypocrisy and the hubris, there is a story here they miss: The city’s current concentration of wealth likely doesn’t represent the beginning of a golden-if-sinful era, but the end.